WT vs MO-grnA injected: 16, 24, 48, 72 hpf trunk region of zebrafish embryos

Transcriptional profiling of zebrafish embryo comparing wild type untreated embryos with embryos injected with morpholino of zf-grna. This assay is used for determination of expression profiling of trunk muscle at 16, 24, 48, 72 hpf under GRN-A deficiency. Two-condition experiment, wild type vs. MO-grnA treated cells. Biological replicates: each group contains 200 embryos.

本数据集聚焦斑马鱼胚胎的转录组分析，对比未处理的野生型斑马鱼胚胎与注射zf-grna吗啉寡聚核苷酸（morpholino）的斑马鱼胚胎。本实验旨在探究GRN-A缺陷条件下，斑马鱼躯干肌肉在受精后16、24、48、72小时（hours post fertilization，hpf）的基因表达谱。本实验采用双条件对照设计：野生型组与MO-grnA处理组样本。生物学重复设置为每组包含200枚斑马鱼胚胎。