Morphology of yellow passion fruit seedlings submitted to triazole induced growth inhibition

ABSTRACT Inhibiting seedling vegetative growth may be a strategy for improving modulation against biotic and abiotic stresses after transplanting. The objective of this study was to evaluate morphological responses of yellow passion fruit (Passiflora edulis) seedlings to growth inhibition resulted from the application of an inhibitor of gibberellin biosynthesis. The experiment was carried out in a greenhouse using seedlings grown in 290 cm3 polyethylene tubes. At 40 days after emergence, trays containing the seedlings were immersed in solutions at concentrations of 0, 40, 80, 120 and 160 mg (Paclobutrazol) PBZ L-1 up to substrate saturation. These five treatments were laid out in a randomized block design with four replicates. At 15, 30 and 45 days after PBZ application (DAA), basal stem diameter was measured; at 45 DAA, stem diameter, individual leaf area, specific leaf area, root diameter and root volume were measured, lignin content and the anatomy of leaves, stems, and roots were analyzed. Triazole-induced anatomical alterations in stems, leaves and roots cause morphological traits and intensify the specific reagent staining of phenolic compounds and lignin in yellow passion fruit (Passiflora edulis) seedlings.

摘要 抑制幼苗营养生长，或可作为提升植株移栽后对生物与非生物胁迫调控抗性的策略。本研究旨在评估黄果西番莲（Passiflora edulis）幼苗经施用赤霉素生物合成抑制剂产生生长抑制效应后的形态学响应。本实验于温室中开展，供试幼苗种植于容积290 cm³的聚乙烯试管内。出苗后40天时，将盛有幼苗的托盘浸入浓度梯度为0、40、80、120、160 mg·L⁻¹多效唑（Paclobutrazol，PBZ）的溶液中，直至基质饱和。该五组处理采用随机区组设计，设置四次重复。分别于多效唑施药后15、30、45天（DAA）测定幼苗基部茎粗；于施药后45天时，测定茎粗、单叶面积、比叶面积、根直径及根体积，并分析木质素含量以及叶片、茎秆与根系的解剖结构。三唑类药剂诱导的茎、叶及根系解剖结构改变，可改变黄果西番莲幼苗的形态性状，并增强其酚类物质与木质素的特异性染色反应。