Expression data from human renal allograft biopsies

Kidney transplants that develop dysfunction or proteinuria after one year post transplant are at considerable risk for progression to renal failure. Identifying the molecules associated with graft failure could potentially lead to interventions that would slow the progression of organ failure. We analyzed the relationship between gene expression in late biopsies for cause in human kidney transplants and subsequent graft loss, and assessed the predictive value of gene expression. We evaluated the performance of these genes in an independent , and in a population of early biopsies that have a very low risk of subsequent graft failure. All consenting renal transplant patients undergoing biopsies for cause as standard of care between 09/2004 and 10/2007 at the university of Alberta or between 11/2006 and 02/2007 at the University of Illinois were included in the analysis. In addition, biopsies obtained from Minnesota between 09/2006 and 09/2007 were used as an independent . In addition to the cores required for standard histopathology, we collected one core for gene expression studies. the relationship between gene expression in the biopsy and subsequent graft loss was analyzed.

肾移植术后一年出现移植肾功能异常或蛋白尿的患者，进展为肾衰竭的风险显著升高。鉴定与移植肾失功相关的分子，有望开发出延缓器官衰竭进展的干预手段。本研究分析了人类肾移植患者因临床指征行晚期移植肾活检的基因表达水平与后续移植肾失功之间的关联，并评估了基因表达的预测价值。我们在独立队列以及后续移植肾失功风险极低的早期活检人群中，评估了上述基因的预测效能。本研究纳入了2004年9月至2007年10月间在阿尔伯塔大学、2006年11月至2007年2月间在伊利诺伊大学，因临床指征接受标准诊疗相关活检且签署知情同意书的肾移植患者。此外，2006年9月至2007年9月间采集自明尼苏达州的活检样本被用作独立队列。除了用于标准组织病理学检测的活检芯组织外，我们还额外采集一份芯组织用于基因表达研究，并分析了活检样本的基因表达水平与后续移植肾失功之间的关联。