Quantitative Assessment of transcriptomic changes induced by aberrant BCL10/MALT1 signaling in primary keratinocytes [CDL]. Quantitative Assessment of transcriptomic changes induced by aberrant BCL10/MALT1 signaling in primary keratinocytes [CDL]

Purpose: The goal of this study is to assess the transcriptomic changes induced by aberrant BCL10/MALT1 signaling downstream of a constitutively active Card11 construct (Rosa26Card11dLinker-lox-STOP-lox x K14Cre) in keratinocytes. Methods: Keratinocytes were isolated from newborn mice, cultured for 5 days and harvested for RNA isolation. mRNA profiles were generated by RNA sequencing using Illumina NextSeq 550. Results: Differential expression analysis identified 293 genes (plus the transgene Card11) to be significantly upregulated (log2 fold change > 1.5, FDR < 0.05) in Card11dLinker expressing keratinocytes compared to those isolated from only KRT14Cre-expressing littermate controls. Overall design: mRNA profiles of in vitro cultured keratinocytes from Card11ΔLinker-K14Cre mice and K14Cre littermate controls

研究目的：本研究旨在评估组成型活性Card11构建体（Rosa26Card11dLinker-lox-STOP-lox x K14Cre）下游异常BCL10/MALT1信号通路诱导的角质形成细胞（keratinocytes）转录组变化。
实验方法：从新生小鼠体内分离角质形成细胞，体外培养5天后收集样本用于RNA提取；采用Illumina NextSeq 550平台进行RNA测序，以生成mRNA表达谱。
实验结果：差异表达分析鉴定出，与仅表达KRT14Cre的同窝对照小鼠分离的角质形成细胞相比，表达Card11dLinker的角质形成细胞中有293个基因（以及转基因Card11）显著上调，筛选标准为log2倍变化（log2 fold change）>1.5、错误发现率（False Discovery Rate, FDR）<0.05。
实验整体设计：对来自Card11ΔLinker-K14Cre小鼠与K14Cre同窝对照小鼠的体外培养角质形成细胞开展mRNA表达谱分析。