URE3 motif in promoters of genes modulated by a dominant positive URE3-BP

Identity (Pathema Locus number) and gene annotation of significantly modulated genes are shown in conjunction with the observed change between induced EF(2)mutURE3-BP and STOP- EF(2)mutURE3-BP strains. The distance between the URE3 motif and the presumed initiating ATG codon is shown as is the position of other potential URE3 motifs, the promoter consensus URE3 motif, and orientation.
1If the motif is present in the promoter in a 5′ to 3′ direction this is indicated by (+) and on the reverse strand by (−).
2These transcripts were decreased in recent clinical isolates [4]
3Was modestly induced by tetracyclin at below threshold values
4These transcripts were increased in recent clinical isolates [4]
5This probe set recognizes a gene shown to be up regulated in HM-1:IMSS compared to a Rahman strain [3]
6A signal peptide was found in either the 5′ extended open reading frame or 3′ of initiating ATG, the position of URE3 motif is shown from the newly designated initiating ATG
7Annotation on the basis of homology to known sulfotransferases
8This transcript was also significantly increased in the EF(2)mutURE3-BPuninduced ameba compared to the uninduced control.
9This gene was described by Nixon et al and has been shown to be up regulated in HM-1:IMSS compared to a Rahman strain [3],[45]

显著调控基因的标识（Pathema基因座编号（Pathema Locus number））与基因注释，结合诱导型EF(2)mutURE3-BP菌株与STOP-EF(2)mutURE3-BP菌株间的观测表达变化一并呈现。同时给出了URE3基序与推定起始ATG密码子之间的距离，以及其他潜在URE3基序、启动子共识URE3基序的位置与方向。
1. 若该基序以5′→3′方向存在于启动子中，以(+)标注；若位于反向链，则以(−)标注。
2. 此类转录本在近年临床分离株中表达下调[4]
3. 该基因在低于阈值浓度的四环素作用下轻度诱导上调
4. 此类转录本在近年临床分离株中表达上调[4]
5. 该探针组识别的基因在HM-1:IMSS株相较于Rahman株中呈上调表达[3]
6. 在5'端延伸的开放阅读框或起始ATG的3'端区域中发现信号肽，此处URE3基序的位置以新指定的起始ATG为基准进行标注
7. 该注释基于与已知磺基转移酶的同源性
8. 相较于未诱导对照组，该转录本在未诱导的EF(2)mutURE3-BP变形虫中同样显著上调
9. 该基因由Nixon等人报道，且已被证实相较于Rahman株，其在HM-1:IMSS株中呈上调表达[3],[45]