Whole genome sequencing of female pompano (Trachinotus ovatus)

Golden pompano (<i>Trachinotus ovatus</i>), belong to the Carangidae family, are important aquaculture fish in China due to their economic value. To aid genomic research in this species, a high-quality reference genome of the golden pompano would be a very useful resource. We produced 106 Gb cleaned Illumina data from paired-end and mate-pair libraries. Another 16.9 Gb long reads data on Pacbio platform were also sequenced. By combination of Illumina and Pacbio reads, we generated a 648 Mb female pompano assembly with a contig N50 size of 1.8 Mb and a scaffold N50 size of 5.05 Mb. With 114.8 Gb cleaned Hi-C data, 99.4% of the total assembled bases were anchored into 24 chromosomes. This assembly would promote the scientific research and aquaculture related to this fish.

卵形鲳鲹（*Trachinotus ovatus*）隶属于鲹科（Carangidae），因其较高的经济价值，是我国重要的海水养殖鱼类。为助力该物种的基因组学研究，构建高质量的卵形鲳鲹参考基因组（reference genome）是极具实用价值的基础资源。本研究从双端文库及mate-pair文库中获取了106 Gb的Clean Illumina测序数据；同时基于PacBio平台完成长读长测序，获得16.9 Gb的测序数据。通过整合Illumina短读长数据与PacBio长读长数据，我们组装得到一个总长度为648 Mb的雌性卵形鲳鲹基因组，其重叠群N50（contig N50）为1.8 Mb，支架N50（scaffold N50）为5.05 Mb。借助114.8 Gb的Clean Hi-C测序数据，我们将99.4%的组装碱基锚定至24条染色体上。该基因组组装成果将有力推动卵形鲳鲹相关的科学研究与水产养殖产业发展。

搜集汇总

背景与挑战

背景概述

该数据集包含雌性金鲳鱼（Trachinotus ovatus）的高质量染色体级别参考基因组，总大小为648 Mb，contig N50为1.8 Mb，scaffold N50为5.05 Mb。数据集还提供了功能注释文件（如GO、KEGG等），为金鲳鱼的基因组研究和养殖提供了重要资源。