SAGE: gene expression in pigs of two commercial lines at the 3 and 9 months og age. Sus scrofa

Muscle development is associated with a modification of mass, composition and function that in productive animals are considered responsible of meat characteristics. In this study, SAGE was employed to describe gene expression in pigs of two commercial lines at the 3 and 9 months og age. The aim was to obtained a full gene expression profile that would assist in identifying genes differentially expressed in the muscle Longissimum dorsi during normal growth. The pattern of expression between lines and ages will help to elucidate the metabolic pathways modulated by these genes during muscle development. Keywords: SAGE, muscle, swine, age comparison, swine comparison Overall design: Twenty castrate pigs, 10 purebred Large White (LW) and 10 Landrace X Duroc (DU), were reared from 3 to 12 months of age in the same environmental and feeding conditions were used. At the age of 3 and 9 month of age, Longissimum dorsi biopsy was collected from each animal of the two groups, using a Magnum Biopsy System (Bard, Covington, GA USA). SAGE libreries were constructed for the biopsies sampled at 3 and 9 from the one Large White and one LandraceXDuroc pig. Animals were selected on the basis of the live weight and daily gains, to have the most “fat” Large White and “lean” LandraceXDuroc.

肌肉发育与肌肉质量、组成及功能的改变密切相关，在商品肉猪中，此类改变被认为是决定肉质特性的核心影响因素。本研究采用基因表达系列分析（Serial Analysis of Gene Expression，SAGE）技术，对两个商业化品系猪在3月龄与9月龄时的基因表达情况进行解析。本研究旨在获取完整的基因表达谱，以辅助鉴定正常生长过程中背最长肌（Longissimum dorsi）内的差异表达基因。不同品系与不同日龄间的基因表达模式，将有助于阐明肌肉发育过程中这些基因所调控的代谢通路。
关键词：基因表达系列分析（SAGE）、肌肉、猪、日龄比较、品系比较
整体实验设计：本研究共选取20头去势公猪，其中10头为纯种大白猪（Large White, LW），剩余10头为长白×杜洛克杂交猪（Landrace × Duroc, DU）；所有试验猪均在一致的环境与饲喂条件下，于3至12月龄阶段饲养。分别在3月龄和9月龄时，采用马格南活检系统（Magnum Biopsy System，巴德公司，美国佐治亚州卡温顿市）采集两组每头猪的背最长肌活检样本。随后，从3月龄和9月龄采样的1头大白猪、1头长白×杜洛克杂交猪的活检样本中构建SAGE文库。试验猪的筛选基于活体重与日增重，以获得脂肪型大白猪与瘦型长白×杜洛克杂交猪。