Mapping the initial effects of carcinogen-induced oncogenic transformation in the mouse bladder

BBN is a bladder specific carcinogen that can cause invasive bladder cancer in mice possessing similar genetic alterations as seen in human cancer. We employed a single cell sequencing approach using 10x Genomics scRNAseq to investigate the cellular heterogeneity and molecular modifications induced by long term treatment with a carcinogen (BBN) on the bladder urothelium and sub-urothelium cells. Our data show infiltrations of B- and T-cells in the tumor and decrease of the several other urothelial populations. 3 sample analysed: 2 non-treated controls and 1 BBN-treated. After 22 weeks of BBN treatment, the mucosa layer of mouse bladder was separated from detrusor and digested to prepare a cell suspension and single live cells were sorted using Fluorescence-activated cell sorting (FACS). Then scRNA-seq was performed to analyze the cells. The same was done for two age-matched non-treated mice.

BBN是一种膀胱特异性致癌物，可在携带与人类癌症相似遗传改变的小鼠中诱导浸润性膀胱癌。本研究采用10x Genomics scRNAseq单细胞测序技术，探究长期给予致癌物BBN处理后，小鼠膀胱尿路上皮及尿路上皮下细胞的细胞异质性与分子修饰变化。本研究数据显示，肿瘤组织中存在B细胞与T细胞浸润，且其余多种尿路上皮细胞群丰度降低。本数据集共分析3份样本：2份未处理对照组样本与1份BBN处理组样本。经22周BBN处理后，我们将小鼠膀胱黏膜层与逼尿肌分离并消化制备细胞悬液，随后通过荧光激活细胞分选（Fluorescence-activated cell sorting, FACS）分选单个活细胞，再对分选得到的细胞开展scRNA-seq分析。对2只同月龄未处理小鼠亦执行相同实验流程。