Leishmania_braziliensis_gp63_functional_domain_sequence 22.fas

DNA sequence of the gp63 fragment encoding the functional domain of leishmanolysin in natural isolates of Leishmania braziliensis from Corte de Pedra, Northeast Brazil: allele 22. The stretch of gp 63 previously described as encoding the functional domain of the molecule was amplified by PCR, cloned into pcr 2.1 vector and bidirectionally sequenced. Sequences from a panel of 41 parasite isolates were aligned and 45 alleles of the fragment could be identified based on indel and SNPs presented among cloned domains. Haplotypes of polymorphisms (indel and SNPs) that could be detected in at least two clones of the gp63 domain in a single isolate, and in at least two parasite isolates in the collection were considered alleles. Corte de Pedra is a region highly endemic for American tegumentary leishmaniasis (ATL) due to L. braziliensis. Several forms of ATL occur and close to 1,500 new cases are diagnosed in the region each year.

巴西东北部Corte de Pedra地区巴西利什曼原虫（Leishmania braziliensis）自然分离株中，编码利什曼蛋白酶（leishmanolysin）功能结构域的gp63片段的DNA序列：等位基因22。此前被报道编码该分子功能结构域的gp63区段经PCR扩增后，被克隆至pcr2.1载体，并完成双向测序。对41株寄生虫分离株的序列进行比对后，基于克隆结构域间存在的插入缺失（indel）与单核苷酸多态性（SNPs），可鉴定出该片段的45种等位基因。若某一多态性的单倍型（插入缺失与单核苷酸多态性）可在单个分离株的至少2个gp63结构域克隆中被检测到，且可在该收集品中的至少2株寄生虫分离株中检出，则将其定义为等位基因。Corte de Pedra地区是巴西利什曼原虫所致美洲皮肤利什曼病（American tegumentary leishmaniasis, ATL）的高流行区。该地区存在多种类型的ATL，每年新确诊病例近1500例。