Combinatorial Approach for Determinant Rate of DNA Sequences Transcription Factors

Research was to study the molecular biology using transcription factor as a determinant of rate of genetic information from DNA to RNA, by binding to a specific sequence. The research question can the genes positioned by regulated by this and traits shown in the appropriate cells and time in the organism. The method involved using a transcriptase where a normal result was negative and a positive result was an indication presence of a pathogen. The combined technique was described quantitative. The results was a combinatorial interaction of transcription factor important for gene regulation. The genomic data sets were relevant to this of each dataset. Recent structure determination of high degree complexes of eukaryotic transcription datasets had residues from their DNA binding regions. These studies supported combinatorial process of transcription regulation in eukaryotic cells and multicellular. It can be concluded existing methods on DNA binding created a transactivator for selection to DNA sequences by guide RNA molecules. The co-traits of this transactivator and combinations of guide RNAs in plant cells.本研究以转录因子（transcription factor）为核心研究对象，旨在探究其通过结合特定核酸序列，调控遗传信息从脱氧核糖核酸（DNA）向核糖核酸（RNA）传递速率的分子生物学机制。本研究的核心科学问题为：该转录因子能否调控其所靶向的基因，以及相关性状能否在生物体的适宜细胞与发育时相中得以表现。本研究采用的实验方法涉及转录酶（transcriptase）体系，其中阴性结果为正常对照，阳性结果则提示病原体的存在。该联合检测技术属于定量分析技术范畴。实验结果表明，转录因子间的组合相互作用对基因调控至关重要。各基因组数据集均与本研究主题相关。近期针对真核转录因子高级复合物的结构解析研究，均捕获到了其DNA结合区域的氨基酸残基信息。这些研究证实了真核细胞及多细胞生物体内转录调控的组合调控机制。综上，现有的DNA结合相关技术已可构建出转录激活因子（transactivator），该因子可借助向导RNA（guide RNA）分子实现对特定DNA序列的靶向筛选。本研究还探究了该转录激活因子与向导RNA组合在植物细胞中所展现的协同性状。