Splicing and gene expression changes in human mammary epithelial MCF-10A cells expressing SR or SR-like proteins

RNA-seq data from human mammary epithelial MCF-10A cells expressing the coding sequence of SRSF2, SRSF4, SRSF6, SRSF9, TRA2B, or a control empty vector plasmid grown for 8 days in 3D culture in matrigel Overall design: We examined the differential gene expression and splicing changes in MCF-10A cells overexpressing SRSF2, SRSF4, SRSF6, SRSF9 or TRA2B compared to control cells.

本数据集的测序样本为经如下处理的人乳腺上皮MCF-10A细胞：分别转染SRSF2、SRSF4、SRSF6、SRSF9、TRA2B的编码序列，或对照空载体质粒，并在基质胶（matrigel）中进行三维培养（3D culture）8天，随后进行RNA测序（RNA-seq）。
整体实验设计：本研究旨在分析过表达SRSF2、SRSF4、SRSF6、SRSF9或TRA2B的MCF-10A细胞与对照细胞之间的差异基因表达及剪接变化。