Lineage tracking example dataset
收藏资源简介：
Example dataset to accompany: Jingzhou Zhang, Justin Griffin, Koushik Roy, Alexander Hoffmann, and Thomas A Zangle, “Tracking of Lineage Mass via Quantitative Phase Imaging and Confinement in Low Refractive Index Microwells,” bioRxiv, doi: 10.1101/2024.03.27.587085. For processing with github.com/Zangle-Lab/Lineage_tracking Dataset contains: - good_well_list.mat: list of wells with single cells at the start of imaging in the example files- good_well_list_full: full list of wells used for processing- dividing_well_list.mat: list of divisions in provided example files- dividing_well_list_full: full list used for processing- QPM20x_4.zip and QPM 20x_10.zip: ZIP files containing QPM20X_LOC_frame_FRAMENUM.mat where LOC = [4,10] and FRAMENUM = [1,2881]: mat files with computed phase shift at each location- QPM20x_10_tif.zip: ZIP file containing QPM20X_LOC_frame_FRAMENUM.mat where LOC = [10] and FRAMENUM = [1,100]: raw tif files from location 10 as examples for the frame alignment code- aligned_images.zip: ZIP file containing cropped and sligned sub-image data from loc4/well26 and loc10/well21- avg_well1.tif and avg_well1.mat: averaged well data used for image alignment- first_frames.zip: ZIP file continaing the first frames (mat and tif tiles) for use in compiling the average well image
本配套示例数据集源自论文：Jingzhou Zhang、Justin Griffin、Koushik Roy、Alexander Hoffmann与Thomas A Zangle发表于预印本平台bioRxiv的《基于定量相位成像（Quantitative Phase Imaging）与低折射率微孔约束的细胞谱系质量追踪》，论文DOI：10.1101/2024.03.27.587085。本数据集用于配合github.com/Zangle-Lab/Lineage_tracking开展处理工作。 数据集包含以下内容： - good_well_list.mat：示例文件中成像起始时刻仅包含单个细胞的微孔列表 - good_well_list_full：用于完整处理流程的微孔全量列表 - dividing_well_list.mat：本次提供的示例文件中记录细胞分裂事件的微孔列表 - dividing_well_list_full：用于完整处理流程的分裂微孔全量列表 - QPM20x_4.zip与QPM 20x_10.zip：内含QPM20X_LOC_frame_FRAMENUM.mat文件的压缩包，其中LOC取值范围为[4,10]，FRAMENUM取值范围为[1,2881]，该MAT格式文件存储了各成像位置的计算相位偏移数据 - QPM20x_10_tif.zip：内含QPM20X_LOC_frame_FRAMENUM.mat文件的压缩包，其中LOC取值为[10]，FRAMENUM取值范围为[1,100]，包含来自位置10的原始TIF图像文件，用作帧对齐代码的示例数据 - aligned_images.zip：包含来自loc4/well26与loc10/well21的裁切配准子图像数据的压缩包 - avg_well1.tif与avg_well1.mat：用于图像对齐的微孔平均图像数据文件 - first_frames.zip：包含首帧图像（MAT格式文件与TIF切片文件）的压缩包，用于编译微孔平均图像
