Supplementary materials for PhD thesis "Temporal genetic diversity in phytoplankton in the Gulf of Naples": Chapter 2 - Library Sequences MC601 and MC597

This dataset comprises the files contained on a CD-ROM which was attached to the thesis when it was submitted in 2006. It was uploaded to ORDO in 2025 for preservation purposes. For more information, please refer to the thesis “Temporal genetic diversity in phytoplankton in the Gulf of Naples" via ORO.Thesis abstractThis thesis dealt with genetic diversity in groups of phytoplankton on a temporal scale. The aim of the study was to investigate diversity and the seasonal distribution of that diversity during the year.Chapter Two examined the diversity and seasonal distribution of ultraplankton <5 μm in surface waters between 2003 and 2004 using 16S class level probes and clone libraries.Chapter Three examined the distribution of ultraplankton using the 16S probes over four depth profiles during 2005. The results showed the presence of Prymnesiophyceae and Cryptophyceae during the whole year and at most depths. The Chrysophyceae showed a marked preference for summer. All three classes showed highest signal just below the surface between 2 and 10 m. The Pelagophyceae were present in February in surface waters, but at 50 to 70 m in May and August. Diversity within these classes was high and other taxonomic classes were detected in the clone libraries. The seasonality within genotypes in the Cryptophyceae was visible and comparisons to known cultures could be made.The diatom genus Pseudo-nitzschia was investigated in Chapter Four using newly developed genus specific primers. Thirteen genotypes were detected during the year, some of which showed seasonal preferences, for example P. multistriata in October, and others that were present most of the year, for example P. galaxiae LSU-clade 2 present from May until October. The three annual blooms of P. galaxiae were investigated in Chapter Five using LSU and ITS. The blooms were shown to be dominated by different ITS genotypes that recur in the same period of the year, every year. The results from P. galaxiae indicated a possible mechanism for speciation whereby populations may become separated in time rather than space.

本数据集包含2006年提交学位论文时随附的光盘只读存储器（CD-ROM）内的全部文件，于2025年上传至ORDO进行馆藏永久保存。如需获取更多信息，请通过ORO查阅学位论文《那不勒斯湾浮游植物的时间尺度遗传多样性》。

论文摘要
本论文围绕浮游植物类群的时间尺度遗传多样性展开研究，旨在探究年度内的物种多样性及其季节分布特征。
第二章采用16S类群水平探针与克隆文库技术，对2003年至2004年表层水体中粒径小于5 μm的超微型浮游植物的多样性与季节分布进行了分析。
第三章于2005年通过四次深度剖面采样，利用16S探针探究了超微型浮游植物的垂直分布特征。研究结果显示：定鞭金藻纲（Prymnesiophyceae）与隐藻纲（Cryptophyceae）全年在绝大多数水深区域均有分布；金藻纲（Chrysophyceae）则显著偏好夏季生境；三个类群的信号峰值均出现在表层以下2~10 m的水层。海囊藻纲（Pelagophyceae）于2月在表层水体中被检出，而在5月与8月则分布于50~70 m水深区域。克隆文库中检出了上述类群内的高多样性类群，同时还检测到其他分类学类群。隐藻纲内基因型的季节分布特征清晰可见，可与已知培养菌株进行比对分析。
第四章利用新开发的属特异性引物，对拟菱形藻属（Pseudo-nitzschia）展开研究。全年共检出13个基因型，其中部分类群表现出明显的季节偏好性，例如多纹拟菱形藻（P. multistriata）仅在10月出现，而另一些类群则全年多数时段存在，例如五月至十月均有分布的galaxiae LSU进化支2。
第五章通过大亚基（LSU）与内转录间隔区（ITS）序列分析，对三次年度拟菱形藻（P. galaxiae）水华事件展开研究。结果表明，每次水华均由同一时期每年重复出现的不同ITS基因型主导。拟菱形藻（P. galaxiae）的研究结果提示了一种潜在的物种形成机制：种群可通过时间隔离而非空间隔离实现分化。