Pipistrellus kuhlii Genome sequencing, assembly and annotation. Pipistrellus kuhlii isolate:mPipKuh1

The Kuhl's pipistrelle (Pipistrellus kuhlii) is a bat species in the family of Vespertilionidae and lives over large areas of North Africa, southern Europe and Western Asia. This assembly has been produced as part of the Bat1K Project. DNA was collected from a male, wild caught in Bergamo, Italy by Emma Teeling and Andrea Locatelli. The samples were prepared in the Long Read team of the DRESDEN concept Genome Center, and sequencing was conducted at DRESDEN concept Genome Center, MPI for Evolutionary Biology, and Phase Genomics. Genome assembly, annotation and manual curation of chromosomes was conducted by the Bat1K group, and in particular by Martin Pippel of Gene Myers lab and David Jebb of Michael Hiller's lab at MPI-CBG. The primary haplotype contains the longest contigs of the DAmar assembly, phased with 10X linked reads, scaffolded with Bionano optical maps, and Phase Genomics HiC reads. Funding was from the Max Planck Society, and individual grants and awards to the persons listed above. The raw data and assembly are currently under a Bat1K publication embargo until removed from this description, following the Bat1K data use policy at the following URL: https://bat1k.ucd.ie/about/

库氏伏翼（Pipistrellus kuhlii）隶属于蝙蝠科（Vespertilionidae），是一种分布于北非、南欧及西亚广阔区域的蝙蝠物种。本次基因组组装为Bat1K计划（Bat1K Project）的研究成果之一。实验所用DNA来自一只雄性野外捕捉个体，该个体由Emma Teeling与Andrea Locatelli在意大利贝加莫捕捉获得。样本制备由德累斯顿概念基因组中心（DRESDEN concept Genome Center）长读长测序团队完成，测序工作则由德累斯顿概念基因组中心、马克斯·普朗克进化生物学研究所（MPI for Evolutionary Biology）以及Phase Genomics共同开展。基因组组装、基因注释及染色体手动注释工作由Bat1K团队完成，其中尤其感谢马克斯·普朗克分子细胞生物学与遗传学研究所（MPI-CBG）Gene Myers实验室的Martin Pippel，以及Michael Hiller实验室的David Jebb。该组装的主要单倍型包含DAmar组装中最长的重叠群（contigs），通过10X连锁reads完成分型，利用Bionano光学图谱与Phase Genomics的Hi-C reads进行支架搭建。本研究的资助方为马克斯·普朗克学会（Max Planck Society），以及上述研究人员获得的个人科研基金与奖项。原始数据与基因组组装目前处于Bat1K计划的论文出版禁运期，直至本说明移除相关内容，具体需遵循Bat1K数据使用政策，相关链接为：https://bat1k.ucd.ie/about/