Time series expression data during phosphate limitation for E. coli K12 JP6015/pMU91. Escherichia coli

A fermentation strategies with phosphate feeding was applied to elongate transition inti phosphate limitation for an tryptophan overproducing E. coli strain High frequency sampling together with the applied fermentation strategy should provide high resolution insights into regulatory regimes involved in phosphate response Overall design: The first sampling timepoint was set as a reference time point after 2,5 h fermentation time to cover phosphate excess conditions. Additional 9 sampling time points were selected based on process dynamics as soon as the phosphate concentrations became sensitive

本研究采用磷酸盐补料发酵策略，以延长色氨酸高产大肠杆菌（E. coli）菌株进入磷酸盐限制环境的过渡阶段。结合高频采样与该发酵策略，可获得磷酸盐响应相关调控机制的高分辨率解析结果。实验总体设计：以发酵2.5小时后的首个采样时间点作为参考时间点，该时间点对应磷酸盐充足条件。当磷酸盐浓度进入敏感区间后，根据发酵过程动态额外选取了9个采样时间点。