CIL: 51800, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library

~1200 cells were imaged on glass. More gradual additions of drug or carrier were carried out in four equal increments at 0, 30, 60, 90 min, together yielding the same final drug concentration indicated in rapid addition studies. The subpopulation of ~100 cells with extended, leading edge pseudopods at t = 0 were imaged and counted in each unit area at the indicated timepoints during a 125 min observation period T=37C

Images are provided as uncorrected, 16 bit .nd2 files to preserve all original data. To view these .nd2 files:
1. Download ImageJ viewer at (https://imagej.nih.gov/ij/download.html)
2. Download the BioFormats.jar package at (https://www.openmicroscopy.org/bio-formats/downloads/)
3. Open the ImageJ directory after installation and drag the extracted BioFormats.jar package into the "Plugins" subdirectory
4. Open an .nd2 image using imageJ and select opening options when prompted, if desired. If no modifications are desired, select "OK"
5. The 16 bit image will typically look dark and will require brightness adjustment.
6. In the ImageJ toolbar, select Image >> Adjust >> Brightness/Contrast and select "Auto", or manually set the brightness and contrast.

我们在玻片上对约1200个细胞进行了成像。实验采用四次等剂量梯度的方式，在第0、30、60、90分钟时逐步添加药物或载体，最终得到的药物终浓度与快速添加实验组一致。在实验初始时刻（t=0）选取约100个具有伸展前沿伪足的细胞亚群，于125分钟的观测周期内（培养温度设定为37℃），在指定时间点对每个单位面积内的该类细胞进行成像与计数。

本数据集提供未经校正的16位.nd2格式图像文件，以保留全部原始数据。若需查看此类.nd2图像文件，请按以下步骤操作：
1. 从以下链接下载ImageJ查看器：https://imagej.nih.gov/ij/download.html
2. 从以下链接下载BioFormats.jar插件包：https://www.openmicroscopy.org/bio-formats/downloads/
3. 安装完成后打开ImageJ安装目录，将解压后的BioFormats.jar拖入其"Plugins"子文件夹中
4. 使用ImageJ打开.nd2图像文件，若有需要可在弹出的提示中选择打开选项；若无需修改参数，直接选择"OK"即可
5. 16位图像通常整体偏暗，需要进行亮度调整
6. 在ImageJ工具栏中依次选择「图像」>「调整」>「亮度/对比度」，可选择"Auto"，或手动调整亮度与对比度。