Peak heights (cps) of the ion chromatograms of steroids metabolites in cell culture medium.

Data are presented as Mean ± SD. (**) presents statistical significance of differences between groups at p<0.01. The peak height values are presented after division by 1000 (cps/1000).
C = steroid metabolites analyzed after 48 h incubation of cell culture in absence of enzyme inhibitors. Apigenin = steroid metabolites analyzed after 48 h of cell culture in the presence of apigenin, the specific inhibitor of 17βHSD. Trilostane = steroid metabolites analyzed after 48 h of incubation of cell culture in the presence of trilostane, the specific inhibitor of 3βHSD. The cell density was approx. 5×106 cells/plate. The experiments were conducted in three independent batches with triplicate repeats for each experiment.
Peak heights (cps) of the ion chromatograms of steroids metabolites in cell culture medium.

数据以平均值±标准差（Mean ± SD）表示。图中标注(**)表示组间差异具有统计学意义（p<0.01）。峰高值已除以1000后进行呈现（单位：cps/1000）。
C组：为无酶抑制剂条件下对细胞培养物孵育48小时后分析得到的类固醇代谢物。芹菜素（Apigenin）组：为在17β-羟类固醇脱氢酶（17βHSD）的特异性抑制剂芹菜素存在下，对细胞培养物孵育48小时后分析得到的类固醇代谢物。曲洛斯坦（Trilostane）组：为在3β-羟类固醇脱氢酶（3βHSD）的特异性抑制剂曲洛斯坦存在下，对细胞培养物孵育48小时后分析得到的类固醇代谢物。细胞接种密度约为5×10^6个细胞/培养皿。本实验共设置3个独立重复批次，每个实验均设置3次技术重复。
本部分展示细胞培养基中类固醇代谢物离子色谱图的峰高（单位：cps）。