Whole-transcriptome analysis in breast cancer with or without lymph node metastasis

To identify the mRNAs, long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs) involved in the pathogenesis of breast cancer, we performed the whole transcriptome sequencing. The whole transcriptome sequencing was performed with three breast cancer tissues with or without lymph node metastasis in each group, respectively. The results showed that 728 mRNAs, 131 lncRNAs, and 144 circRNAs were differentially expressed in lymph node metastasis group and no lymph node metastasis group (fold change≥2, p<0.05). These data indicate that dysregulation of mRNAs, lncRNAs, and circRNAs may contribute to breast cancer progression. We performed the whole-transcriptome sequencing in six cases of breast cancers, including three cases with lymph node metastasis(M) and three cases without lymph node metastasis(N), and identified the expression profiles of mRNAs, lncRNAs, and circRNAs.

为鉴定参与乳腺癌发病机制的信使RNA（mRNAs）、长链非编码RNA（long non-coding RNAs，简称lncRNAs）以及环状RNA（circular RNAs，简称circRNAs），本研究开展了全转录组测序实验。本实验每组分别设置3例伴或不伴淋巴结转移的乳腺癌组织作为样本进行全转录组测序。结果显示，淋巴结转移组与非淋巴结转移组间共有728个mRNAs、131个lncRNAs以及144个circRNAs存在差异表达（倍数变化≥2，p<0.05）。上述数据表明，mRNAs、lncRNAs及circRNAs的表达失调可能参与乳腺癌的进展过程。本研究对6例乳腺癌患者样本进行了全转录组测序，其中3例为伴淋巴结转移（M）病例，3例为无淋巴结转移（N）病例，并成功鉴定出上述三类RNA的表达谱。