Ubiquitination of hepatic APOC3 by GM47544 prevents hypertriglyceridemia and ameliorates atherosclerosis

To investigate the function of lnc GM47544 in the regulation of triglyceride, we established AML-12 cell lines in which lnc GM47544 has been stably overexpressed by lentivirus. We then performed gene expression profiling analysis using data obtained from RNA-seq of AML-12-pLKO.1 (control) and AML-12-GM47544 (O/E). Comparative gene expression profiling analysis of RNA-seq data for AML-12-pLKO.1 (control) and AML-12-GM47544 (O/E).

为探究长链非编码RNA（long non-coding RNA，lnc）GM47544在甘油三酯调控中的功能，我们通过慢病毒构建了稳定过表达lnc GM47544的AML-12细胞系。随后，我们针对AML-12-pLKO.1（对照组）与AML-12-GM47544（过表达组，O/E）的RNA测序（RNA-seq）数据开展基因表达谱分析。本研究针对AML-12-pLKO.1（对照组）与AML-12-GM47544（过表达组，O/E）的RNA-seq数据进行了对比基因表达谱分析。