Expression analysis of circadian light response in Drosophila recombinant inbred lines (RIL)

The aim of this study was to identify quantitative trait loci (QTL) associated with variation in circadian photosensitivity in Drosophila, by measuring the locomotor response to early night light pulse (Zt15). We used QTL mapping of recombinant inbred lines (RIL), which was followed by microarrays expression comparison of two RI lines which differed significantly in their light response (RIL 104 shows a strong long phase delay response while RIL 58 shows a weak light response). Each of the two strains was sampled after light pulse, and without a light pulse (control), totalling 8 samples: 2x lines (L) x treatments (T) x 2 replicates

本研究旨在通过测定果蝇对夜间早期光脉冲（Zt15）的运动响应，鉴定与果蝇昼夜光敏感性变异相关的数量性状位点（QTL）。本研究采用重组自交系（RIL）开展QTL定位，随后选取两个光响应差异显著的重组自交系进行基因芯片表达谱比较：其中RIL 104呈现出强烈的长相位延迟响应，而RIL 58仅表现出微弱的光响应。我们对这两个品系分别设置光脉冲处理组与无脉冲对照组，每组均设置2次生物学重复，总计8个样本（2个品系×2种处理×2次重复）。