Gene expression profile at single cell level of leukocytes from the hearts of 5-day-old mice that underwent apical resection and Triiodothyronine treatment

To investigate the mechanism of Triiodothyronine regulating neonatal mice heart regeneration, we performed apical resection (AR) surgery on postnatal day 1 (P1) CD1 mice and administered with T3 (20ng/g body weight) or its solvent, PBS as control group. We harvested AR-injured hearts and isolated CD45+ leukocytes after T3 administration at 4 day-post-resection (dpr) .

为探究三碘甲状腺原氨酸（Triiodothyronine）调控新生小鼠心脏再生的机制，本研究对出生后第1天（P1）的CD1品系小鼠实施心尖切除（apical resection, AR）手术，并于切除术后第4天（day-post-resection, dpr）分别给予20ng/g体重的T3及其溶剂磷酸盐缓冲液（PBS）作为对照组；给药完成后，我们收集了心尖切除损伤的心脏，并分离得到CD45阳性白细胞。