Expression analysis of Danio rerio semaphorin6a and plexinA2 morpholino-injected embryos. Danio rerio

Investigation of whole genome gene expression level changes in Danio rerio semaphorin6A and plexinA2 morphant animals, compared to the wild-type strain. The morphants analyzed in this study are further described in Ebert AM, Childs SJ, Hehr C, Cechmanek PB, McFarlane S. 2014. Sema6a and Plxna2 mediate spatially regulated repulsion within the developing eye to promote eye vesicle cohesion. Development 141(12):2473-2482. Overall design: A twelve chip study using total RNA recovered from four replicates of control, semaphorin6a morpholino-injected and plexinA2 morpholino-injected 18 hour postfertilization rx3:GFP zebrafish embryos. Samples were run on a NimbleGen Zebrafish Gene Expression 385K Microarray

本研究旨在探究斑马鱼（Danio rerio）信号素6A（semaphorin6A）与丛状蛋白A2（plexinA2）吗啉代敲低个体（morphant）的全基因组基因表达水平变化，并以野生型品系作为对照进行对比分析。本研究中所分析的敲低个体的详细信息，已在Ebert AM、Childs SJ、Hehr C、Cechmanek PB、McFarlane S等人2014年发表的论文中进一步阐述：《Sema6a与Plxna2介导发育中眼睛内的空间调控排斥反应以促进视泡凝聚》，刊载于《发育（Development）》期刊第141卷第12期，页码范围为2473-2482。实验整体设计：本研究包含12张芯片的检测流程，所使用的总RNA提取自受精后18小时的rx3:GFP转基因斑马鱼胚胎的四组生物学重复样本，样本分为对照组、信号素6A吗啉寡核苷酸注射组以及丛状蛋白A2吗啉寡核苷酸注射组。所有样本均在NimbleGen斑马鱼基因表达385K微阵列（NimbleGen Zebrafish Gene Expression 385K Microarray）上完成检测。