Characterization and Sequencing of Pathogenic Fungi Pestalotiopsis vismiae and Antagonistic Yeast Metschnikowia pulcherrima E1 for Loquat Gray Spot Disease Research

This project aims to deposit nucleotide sequences of the pathogenic fungus Pestalotiopsis vismiae and the antagonistic yeast Metschnikowia pulcherrima E1 into NCBI. P. vismiae was isolated from postharvest decaying loquat fruits in Zhenjiang, while M. pulcherrima E1 was screened from loquat leaves and soil. genomic DNA was extracted using a fungal DNA kit (Tiangen), and PCR amplification of the ITS-5.8S rDNA region was performed under standard conditions (95C pre-denaturation for 30 s, 40 cycles of 95C/5 s, 62C/30 s, 72C/20 s) followed by Sanger sequencing (Nanjing Jisi Huiyuan Biological Co., Ltd.). These sequences are crucial for taxonomic validation of P. vismiae (confirmed via 99% identity with GenBank MH855246) and M. pulcherrima E1, and they underpin transcriptomic findings showing yeast-induced resistance pathways in loquat fruits (e.g., upregulation of CML19, GSTU10). The data will facilitate molecular detection of pathogens and mechanistic studies on yeast-mediated defense, including roles in plant-pathogen interactions, secondary metabolism, and glutathione metabolism.

本项目旨在将致病真菌*Pestalotiopsis vismiae*及拮抗性酵母*Metschnikowia pulcherrima* E1的核苷酸序列提交至美国国家生物技术信息中心（National Center for Biotechnology Information, NCBI）。*P. vismiae*分离自镇江地区采后腐烂的枇杷果实，而*M. pulcherrima* E1则从枇杷叶片及土壤中筛选获得。研究人员采用真菌基因组DNA提取试剂盒（天根生化，Tiangen）提取基因组DNA，通过标准聚合酶链式反应（Polymerase Chain Reaction, PCR）体系扩增ITS-5.8S核糖体DNA（rDNA）区域：95℃预变性30秒，随后以95℃变性5秒、62℃退火30秒、72℃延伸20秒进行40个循环，最终由南京基思惠源生物科技有限公司（Nanjing Jisi Huiyuan Biological Co., Ltd.）完成桑格测序（Sanger sequencing）。上述序列对于*P. vismiae*及*M. pulcherrima* E1的分类学验证具有关键意义：经序列比对，二者与基因银行（GenBank）登录号MH855246的序列相似度达99%，从而确认了其分类地位；同时，该数据为阐释酵母诱导枇杷果实抗病通路的转录组学研究提供了核心支撑，例如CML19、GSTU10基因的上调表达。本数据集将为病原菌的分子检测以及酵母介导的植物防御机制研究提供助力，相关研究方向涵盖植物-病原菌互作、次生代谢以及谷胱甘肽代谢等领域。