Comparison of gene expression between MEFs, MEFs (NWD), immature Sertoli cells and mature Sertoli cells. Mus musculus

To compare the the genomic profile of MEFs, immature Sertoli, mature Sertoli cells, and MEFs (NWD) after infection of Nr5a1, Wt1 and Dmrt1 after 1 month of dox exposure. Overall design: MEFs (NWD) were infected with Nr5a1, Wt1 and Dmrt1 and were exposed to dox for 1 month. MEFs, immature Sertoli and mature Sertoli cells were cultured for 3 days and collected

本研究旨在比较感染Nr5a1、Wt1及Dmrt1基因并经1个月多西环素（doxycycline, dox）诱导后，小鼠胚胎成纤维细胞（Mouse Embryonic Fibroblasts, MEFs）、未成熟支持细胞、成熟支持细胞以及MEFs (NWD)的基因组图谱。整体实验设计：将MEFs (NWD)感染Nr5a1、Wt1及Dmrt1基因后，施以1个月的多西环素诱导；同时将MEFs、未成熟支持细胞与成熟支持细胞培养3天后收集样本。