GSE47295: HP2 (Ab2-90).D.mel 3rd Instar Larvae Nuclei.Solexa

Summary: modENCODE_submission_5113 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of 125 chromosomal proteins across the Drosophila melanogaster genome. The proteins under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using the Illumina NGS platform. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Overall Design: EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Strain: Oregon-R(official name : Oregon-R-modENCODE genotype : wild type ); Developmental Stage: 3rd Instar Larvae; Genotype: wild type; EXPERIMENTAL FACTORS: Strain Oregon-R(official name : Oregon-R-modENCODE genotype : wild type ); Antibody HP2 (Ab2-90) (target is HP2); Developmental Stage 3rd Instar Larvae

摘要：modENCODE_submission_5113
本提交数据源自Gary Karpen主持的modENCODE项目。如需查阅modENCODE项目完整列表，请访问http://www.genome.gov/26524648。
项目目标：本项目拟定位黑腹果蝇（Drosophila melanogaster）基因组内的125种染色体蛋白质。本次研究涉及的蛋白质参与DNA复制、基因表达、基因沉默与遗传等核心染色体生物学功能。本研究将依托Illumina NGS平台开展染色质免疫沉淀（Chromatin ImmunoPrecipitation, ChIP）实验。研究初期将利用3种细胞系及2个胚胎阶段的染色质完成蛋白质定位检测，后续将把部分蛋白质的分析范围扩展至另外4种动物组织/发育阶段。数据使用条款与规范请参阅http://www.genome.gov/27528022及http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。

实验设计：
实验类型：染色质免疫沉淀测序（ChIP-seq）
生物样本来源：品系：Oregon-R（官方命名：Oregon-R-modENCODE，基因型：野生型）；发育阶段：三龄幼虫；基因型：野生型；
实验因素：品系：Oregon-R（官方命名：Oregon-R-modENCODE，基因型：野生型）；抗体：HP2（Ab2-90）（靶标为HP2）；发育阶段：三龄幼虫