RNA-seq analysis of MPCs treated with FGF1 variants

RNA-seq is a powerful tool to analyze differential expression of cellular pathways under different conditions. The goal of this study is to analyze the potential pathways involved in cellular defense against high glucose challenge with or without FGF1 intervention. Overall design: MPC cells were starved for 12 hours in serum-free RPMI-1640 medium and pretreated with FGF1 wild type or FGF1 variant (100 ng/mL) for 1 hour. Then, the cells were challenged with high glucose (25 mM) (with D-mannitol as an osmotic control) for 12 hours. After that, cells were harvested for RNA-seq analysis.

RNA测序（RNA-seq）是用于分析不同条件下细胞通路差异表达的强有力研究工具。本研究旨在探究参与细胞抵御高葡萄糖刺激（施加或不施加成纤维细胞生长因子1（FGF1）干预）的潜在细胞通路。实验整体设计如下：将MPC细胞在无血清RPMI-1640培养基中饥饿12小时，随后用野生型FGF1或FGF1变体（100 ng/mL）预处理1小时；继而以高葡萄糖（25 mM）处理细胞（以D-甘露醇作为渗透压对照），刺激时长为12小时，最后收集细胞开展RNA-seq分析。