Impact of embryonically-derived Kupffer cells depletion on liver gene expression under hypercholesterolemic conditions. Impact of embryonically-derived Kupffer cells depletion on liver gene expression under hypercholesterolemic conditions

Kupffer cells (KCs) are the tissue resident macrophage population of the liver. With this experiment, we sought to investigate the potential consequences of decreasing embryonically-derived KC numbers on liver gene expression in the context of hypercholesterolemia. 3-month-old CD207-DTR x LDL-receptor deficient (C57BL6J background) female mice were injected with diphtheria toxin (DT) or heat-inactivated DT. Mice were then fed a cholesterol-rich diet to induce hypercholesterolemia. RNA sequencing was performed on liver RNA samples after 4 weeks of diet.

库普弗细胞（Kupffer cells, KCs）是肝脏的组织驻留巨噬细胞群体。本实验旨在探究高胆固醇血症背景下，胚胎起源的库普弗细胞数量减少对肝脏基因表达的潜在影响。将背景品系为C57BL/6J的3月龄CD207-DTR × 低密度脂蛋白受体缺陷雌性小鼠，分别注射白喉毒素（diphtheria toxin, DT）或热灭活白喉毒素。随后给小鼠喂食高胆固醇饲料以诱导高胆固醇血症，造模4周后对肝脏RNA样本开展RNA测序。