TP53 promotes lineage commitment of human embryonic stem cells through ciliogenesis and sonic hedgehog signaling [RNA-Seq]

H9 WT ESCs or TP53 KO (Clone 1) ESCs were injected subcutaneously into mice for teratoma formation. 6-8 weeks later, teratomas were isolated and total RNA was purified. TruSeq library preparation kit from Illumina was used to make libraries for RNA-Seq. NGS sequencing on Illumina NextSeq 500 was performed and data was analyzed to determine changes in transcriptome between WT and TP53 KO teratoma cells. Transcriptome of WT and TP53 KO teratoma cells.

将H9野生型胚胎干细胞（embryonic stem cells, ESCs）或TP53基因敲除（克隆1）胚胎干细胞（TP53 KO (Clone 1) ESCs）皮下注射至小鼠体内以诱导畸胎瘤形成。6-8周后，分离畸胎瘤并纯化总RNA。采用Illumina公司的TruSeq文库制备试剂盒构建RNA测序（RNA-Seq）文库，在Illumina NextSeq 500测序平台上完成下一代测序（next-generation sequencing, NGS），随后对测序数据进行分析，以明确野生型与TP53基因敲除畸胎瘤细胞之间的转录组差异。本数据集涵盖野生型及TP53基因敲除畸胎瘤细胞的转录组信息。