Underlying and Extended Data for Refined and benchmarked homemade media for cost-effective, weekend-free human pluripotent stem cell culture

Extended Data and raw data for the manuscript "Refined home-brew media for cost-effective, weekend-free hiPSC culture and genetic engineering" Extended Data 1.zip - protocol for preparation of the supplement for hE8 and B8+ media Extended Data 2.zip - Gene counts, supporting files, and output results of bulk analyses Extended Data 3.zip - Images of iPS cells adapted to cE8, hE8 and B8+ taken 24, 48 and 72 hours after passage. Contains raw .tiff files for each image and a .pdf with a compiled figure Extended Data 4.zip - Results of miloR analysis on the differences in the distribution of cells adapted to cE8, hE8 and B8+ to assigned monocle clusters Manuscript Data.zip – Raw data underlying the Figures 1, 2, 4, 5, 6 and 7. Bulk_RNA_seq_archive – archived source code used for generating results in Figure 3本数据集对应论文《优化自制培养基：实现低成本、无需周末值守的人诱导多能干细胞（human induced pluripotent stem cells, hiPSC）培养与基因工程》的补充数据与原始数据。 补充数据集1.zip：hE8与B8+培养基补剂的制备方案 补充数据集2.zip：批量分析的基因计数数据、辅助文件与输出结果 补充数据集3.zip：细胞传代后24、48、72小时时，适配cE8、hE8与B8+培养基的诱导多能干细胞成像图，包含所有图像的原始.tiff格式文件，以及一份包含整合图表的.pdf文件 补充数据集4.zip：针对适配cE8、hE8与B8+培养基的细胞在Monocle聚类中的分布差异进行miloR分析的结果 论文原始数据.zip：支撑论文图1、2、4、5、6、7的原始数据 批量RNA测序存档.zip：用于生成图3结果的源代码存档