Effects of dietary yogurt consumption on the gastrointestinal (GI) microbial communities of healthy human subjects. human gut metagenome

The aim of this study was to determine the microbial community composition of healthy human test subjects consuming commercially available yogurt for 42 consecutive days. Terminal restriction fragment length polymorphism, Sanger sequencing, and next generation sequencing were used in to characterize changes in microbial community structure, diversity, and phylogenetics of eubacteria, more specifically Lactobacilli and Bifidobacteria. We hypothesized that introducing a yogurt diet to human test subjects would: i) alter microbial community structure, ii) increase GI microbial diversity and iii) increase the abundance of probiotic bacteria.

本研究旨在探究连续42天食用市售酸奶的健康人类受试者的微生物群落组成。本研究采用末端限制性片段长度多态性（Terminal Restriction Fragment Length Polymorphism）、桑格测序（Sanger Sequencing）以及下一代测序（Next Generation Sequencing）三种技术，对真细菌（Eubacteria，其中更具体聚焦于乳杆菌属（Lactobacilli）与双歧杆菌属（Bifidobacteria））的群落结构、多样性及系统发育特征的变化进行表征。我们提出如下研究假设：向人类受试者引入酸奶饮食后，将产生以下三类效应：i）改变微生物群落结构；ii）提升胃肠道（Gastrointestinal）微生物群落多样性；iii）增加益生菌的丰度。