RNA-Sequencing of STING competent and STING-KO cells after transfection with dacA (Listeria monocytogenes)

HEK-Blue™ ISG Cells and HEK-Blue™ ISG-KO-STING Cells (Invivogen) were transfected with dacA (Listeria monocytogenes) for 12 and 24 hours. Through this approach, we described the STING-dependent and STING-independent transcriptional response to dacA. Our analysis reveals a STING-dependent enrichment of interferon stimulated genes beginning 12 hours post-transfection, and a STING-independent enrichment of genes associated with protein translation and oxidative phosphorylation. Transcriptional responses to dacA transfection in STING-WT and STING-KO cells.

本研究采用Invivogen公司的HEK-Blue™ ISG细胞与HEK-Blue™ ISG-KO-STING细胞，以单核细胞增生李斯特菌来源的dacA基因进行转染，分别处理12小时与24小时。通过该实验方案，我们解析了dacA刺激下的STING依赖型与STING非依赖型转录应答。分析结果表明，转染后12小时即可检测到STING依赖型干扰素刺激基因（interferon stimulated genes，ISG）的富集；而与蛋白质翻译、氧化磷酸化相关的基因则呈现STING非依赖型富集。本研究同时对STING野生型与STING敲除型细胞中dacA转染引发的转录应答进行了分析。