2017-2018年柔性非晶结构的仿生丝蛋白支架的设计及探究其生物相容性研究数据

以非晶丝蛋白纳米短纤维溶液为基元，一方面，利用温度诱导调控丝蛋白的自组装过程，制备出力学性能在1-4KPa可精细调控的水稳定性丝蛋白支架，并研究了其基本性能和生物相容性。结果表明通过动力学因素控制，可主动改变多孔海绵的二级构象组成、模量以及降解性，体内和体外结果均表现出良好的细胞和组织相容性，可应用于软组织修复领域。另一方面，通过甘油和温度诱导进一步降低丝蛋白支架的力学性能，应用到神经组织修复,并对其二级结构、降解性及力学性能等进行研究。实验结果表明，甘油和温度诱导可制备出力学性能低于1KPa的水稳定性丝蛋白支架，以期更好地应用于神经组织的修复。Using solutions of amorphous short silk protein nanofibers as the foundational building block, on one hand, we regulated the self-assembly process of silk proteins via temperature induction, prepared water-stable silk protein scaffolds with mechanical properties finely tunable within 1–4 KPa, and investigated their basic properties and biocompatibility. Results demonstrated that controlling kinetic factors can actively adjust the secondary conformation composition, modulus, and degradability of the porous spongy scaffolds. Both in vivo and in vitro assays exhibited excellent cellular and tissue biocompatibility, rendering these scaffolds suitable for soft tissue repair applications. On the other hand, we further reduced the mechanical properties of silk protein scaffolds through glycerol- and temperature-induced treatment, applied these scaffolds to nerve tissue repair, and conducted systematic studies on their secondary structure, degradability, mechanical properties, and other key characteristics. Experimental results showed that this glycerol- and temperature-induced fabrication approach can produce water-stable silk protein scaffolds with mechanical properties below 1 KPa, with the goal of enabling better application of these scaffolds in nerve tissue repair.