miRNAs expression profile in bone marrow-derived PACs during ischemia-induced neovascularization in cardiovascular risk factors conditions

Using NGS, we report the impact of cardiovascular risk factors (CRF) including hypercholesteolemia, aging and smoking compare to healthy control mice, on the differential-expression profile of miRNAs in proangiogenic cells (PACs) isolated during ischemia-induced neovascularization in a mouse model of hindlimb ischemia. Data of total miRNA expression are presented as normalized copy number: read per millions of read (RPM). Hindlimb ischemia was surgicaly induced by feromal artery ablation and the animal was sacrified 2 day after ischemic surgery for PACs isolation. After 4 days of culture, PACs RNAs were extracted to perform NGS. Overall design: Examination of the impact of CRF on miRNAs expression profile and modulation, in isolated PACs during ischemia-induced nerovascularization.

本研究借助下一代测序（Next Generation Sequencing, NGS），针对后肢缺血小鼠模型中缺血诱导血管新生阶段分离得到的促血管生成细胞（proangiogenic cells, PACs），分析包括高胆固醇血症、衰老与吸烟在内的心血管危险因素（cardiovascular risk factors, CRF）相较于健康对照小鼠，对其微小RNA（miRNAs）差异表达谱的影响。总微小RNA表达数据以归一化拷贝数形式呈现，单位为每百万读段数（reads per million, RPM）。后肢缺血模型通过股动脉消融手术诱导构建，于缺血手术后2天处死小鼠以分离PACs。经4天体外培养后，提取PACs的总RNA并开展下一代测序。整体实验设计：考察心血管危险因素对缺血诱导血管新生阶段分离得到的PACs中miRNAs表达谱及其调控模式的影响。