尘肺病人和非尘肺供体的qPCR统计数据

收集肺移植手术中尘肺患者和供体的肺组织，提取RNA，反转录成cDNA，用qPCR检测炎性基因TNF-α、IL-1β、IL-6以及纤维化基因COL-1、FN的相对表达量。根据实验结果评估这些基因是否可以作为评估矽肺发病的指标。这些结果也可以提示肺移植患者的炎症水平和纤维化水平。Collect lung tissues from silicosis patients and donors during lung transplantation surgery, extract RNA, reverse transcribe into cDNA, and quantify the relative expression levels of inflammatory genes TNF-α, IL-1β, IL-6 and fibrotic genes COL-1, FN via qPCR. Based on the experimental results, assess whether these genes can serve as indicators for the pathogenesis of silicosis. These findings can also reflect the inflammatory and fibrotic status of lung transplant recipients.