Metabarcoding with 16S and COI rRNA gene of sediments involved in the digestive processes of three tropical sea cucumber species for Holomicro project, 2019. Holomicro

Study has focused on bacteria, archea and eukaryota found in the sediment (beneath tentacles), along the digestive tract and in faeces of three sea cucumber species in five stations across two coral reefs of Reunion Island (French Island, Southern Indian Ocean) during three consecutive seasons. PCR primers used were: 16S rRNA V4-V5 region: 515F : 5-GTYCCAGCMGCCGCGGTAA-3 ; 928R : 5-CCCCGYCAATTCMTTTRAGT-3; 16S rRNA Archea: 519F : 5'-CAGCMGCCGCGGTAA-3' ; 915R : 5'-GTGCTCCCCCGCCAATTCCT-3' ; and COI: m1COIintF-XT : 5'-GGWACWGGWTGAACWGTWTAYCCYCC-3' ; jgHCO2198 : 5'-TAIACYTCIGGRTGICCRAARAAYCA-3'.

本研究聚焦于位于南印度洋的法国海外领地留尼汪岛的两处珊瑚礁的五个采样站点，于连续三个季节中，对三种海参物种触手下方的沉积物、消化道以及粪便内的细菌、古菌与真核生物展开了相关研究。本次实验所用的PCR引物如下：针对16S rRNA V4-V5区域的引物为515F：5'-GTYCCAGCMGCCGCGGTAA-3'；928R：5'-CCCCGYCAATTCMTTTRAGT-3'；针对古菌16S rRNA的引物为519F：5'-CAGCMGCCGCGGTAA-3'；915R：5'-GTGCTCCCCCGCCAATTCCT-3'；针对COI基因的引物为m1COIintF-XT：5'-GGWACWGGWTGAACWGTWTAYCCYCC-3'；jgHCO2198：5'-TAIACYTCIGGRTGICCRAARAAYCA-3'。