Treatment of CHRF288-11 Megakaryocytic Cells with Wnt compounds

CHRF288-11 cells were treated with Wnt3a (150ng/ml) and/ot Wnt5a (1200ng/ml) and RNA was harvested after 8 hours of treatment Gene expression was analysed using Human WG6 V3 Illumina Beadarrays There are four experimental groups (Wnt3a Treated, Wnt5a Treated, Wnt3a + Wnt5a treated and Untreated (control)), each with three biological replicates

将CHRF288-11细胞以150ng/ml的Wnt3a以及（或）1200ng/ml的Wnt5a进行单独或联合处理，于处理8小时后收集RNA。采用人类WG6 V3版Illumina Beadarrays微珠芯片对基因表达水平进行分析。本实验共设置四组实验组：Wnt3a处理组、Wnt5a处理组、Wnt3a与Wnt5a联合处理组以及未处理对照组，每组均包含3次生物学重复。