Pulmonary effects of ozone therapy at different doses combined with antibioticotherapy in experimental sepsis model

Abstract Purpose This experimental sepsis model created with Escherichia coli aimed to investigate the histopathological effects of two different doses of ozone combined with antibiotherapy on lung tissue. Methods Rats were divided into 5 groups. Then sepsis was induced intraperitoneally in the first 4 groups. The 1st group was treated with cefepime, the 2nd and 3rd groups were treated with cefepime combined with ozone at a dose of 0.6 mg/kg and 1.1 mg/kg. Lung tissue sections were stained with hematoxylin-eosin and assessed under light microscope and scored between 0-4 in terms of histopathological findings. Results In the comparisons between Group 1 and Group 4 in terms of cellular damage (p=0.030), inflammation (p=0.000) and overall score (p=0.007), statistically significant positive effects were observed in favor of Group 1. In the comparisons of Groups 2 and 3 with Group 4, only positive effects were observed in terms of inflammation (p=0.020, p=0.012, respectively). Conclusion Although negative histopathological effects of ozone on tissue injury were detected, it was noteworthy that the increase in the ozone dose reduced the number of damaged parameters.

摘要
研究目的：本研究采用大肠杆菌构建实验性脓毒症模型，旨在探讨两种不同剂量臭氧联合抗生素治疗对肺组织的组织病理学影响。
研究方法：将大鼠分为5组，前4组经腹腔注射诱导脓毒症。第1组予以头孢吡肟单药治疗；第2、3组分别予以头孢吡肟联合0.6mg/kg、1.1mg/kg臭氧治疗。取肺组织切片行苏木精-伊红（hematoxylin-eosin）染色，于光学显微镜下观察并根据组织病理学表现进行0~4分评分。
研究结果：第1组与第4组相比，在细胞损伤（p=0.030）、炎症反应（p=0.000）及总病理评分（p=0.007）方面均观察到具有统计学意义的正向改善效果，且以第1组为优。第2、3组分别与第4组比较时，仅在炎症反应维度呈现出具有统计学意义的正向改善效果（对应p值分别为0.020、0.012）。
研究结论：尽管本研究检测到臭氧对组织损伤存在负面的组织病理学影响，但值得关注的是，臭氧剂量升高可减少受损参数的数量。