Draft genome of Mammaliicoccus sciuri isolated from street foods in Bangladesh

A Staphylococcus sciuri isolate, currently identified as Mammaliicoccus sciuri was isolated from street food samples gathered in Mymensingh, Bangladesh, as part of a public health surveillance initiative program. The coagulase-negative staphylococcal species Mammaliicoccus sciuri is becoming more widely known for its involvement in human opportunistic illnesses and antibiotic resistance. Due to the high risk of cross contamination and the widespread consumption of street food in crowded metropolitan areas, foodborne transmission of these resistant bacteria poses a serious health risk. Standard microbiological techniques were used to isolate bacteria from samples obtained from different street vendors. We extracted genomic DNA from pure cultures and sequenced it using the Illumina NextSeq 2000 platform with paired-end 150 bp reads at about 50X coverage. Several antimicrobial resistance genes, including those linked to beta-lactam resistance and efflux processes, were discovered through genome assembly and annotation. Additionally, a number of genes linked to virulence were found, indicating possible pathogenicity. This dataset provides useful genetic data on Mammaliicoccus sciuri from a low-resource, non-clinical, food-associated source. These results emphasize how crucial it is to keep an eye out for bacterial contamination in street food and the potential health risks. Future research on the molecular epidemiology, resistance profiling, and phylogenetic relationships of Mammaliicoccus sciuri and related species will be aided by the submitted WGS data.

本研究作为公共卫生监测项目的一部分，从孟加拉国迈门辛市采集的街头食品样本中分离出一株原鉴定为松鼠葡萄球菌（Staphylococcus sciuri）、现归类为松鼠马莫里球菌（Mammaliicoccus sciuri）的菌株。作为凝固酶阴性葡萄球菌类群之一，松鼠马莫里球菌因其可引发人类机会性感染并产生抗生素耐药性，正日益受到广泛关注。鉴于人口密集的大都市区域内街头食品消费场景普遍且交叉污染风险极高，这类耐药菌的食源传播会带来严重的健康隐患。研究采用标准微生物学技术，从不同街头摊贩采集的样本中分离得到目标细菌；随后从纯培养物中提取基因组DNA，使用Illumina NextSeq 2000测序平台开展双端150碱基对（bp）测序，测序覆盖度约为50倍。经基因组组装与注释分析，研究发现了多个抗菌耐药基因，包括与β-内酰胺类耐药及外排泵机制相关的耐药基因；此外还鉴定出多个与毒力相关的基因，提示该菌株具备潜在致病性。本数据集提供了来自资源匮乏地区、非临床且与食品相关来源的松鼠马莫里球菌的宝贵遗传数据。本研究结果凸显了监测街头食品细菌污染及相关潜在健康风险的重要性。本次提交的全基因组测序（Whole Genome Sequencing, WGS）数据，将为后续针对松鼠马莫里球菌及其近缘物种的分子流行病学、耐药谱分析及系统发育关系研究提供有力支撑。