RNA sequencing of different GEMMs of prostate cancer

Prostate cancers of different genetic backgrounds were subjected to polysome profiling analysis to identify the extracellular interactome between prostate cancer and myeloid-derived suppressor cells. Polysome profiles analysis of prostate was performed in six different genetic backgrounds: wild-type, Ptenpc-/-, Ptenpc-/-;TMPRSS2-ERGpc+/+, Ptenpc-/-;CDCP1pc+/+, Ptenpc-/-;TIMP1-/- and Ptenpc-/-;Trp53pc-/-. Polysome profiling of the prostate of three mice for genotype was performed.

为鉴定前列腺癌与髓系来源抑制细胞（myeloid-derived suppressor cells）之间的细胞外互作组（extracellular interactome），本研究对携带不同遗传背景的前列腺癌样本开展了多聚核糖体谱分析（polysome profiling analysis）。本次针对前列腺组织的多聚核糖体谱分析共涵盖6种遗传背景组别：野生型（wild-type）、Ptenpc-/-、Ptenpc-/-;TMPRSS2-ERGpc+/+、Ptenpc-/-;CDCP1pc+/+、Ptenpc-/-;TIMP1-/- 以及 Ptenpc-/-;Trp53pc-/-。针对每种基因型，本研究均选取3只小鼠的前列腺组织完成多聚核糖体谱分析。