Genome-wide CUT&RUN sequencing of H3K27me3 mark across different stages of T-cell development.

We present CUT&RUN sequencing of H3K27me3 for hematopoietic progenitor cells at the following differentiation stages: 1) cKit+ hematopoietic progenitor cells from bone marrow, 2) monoallelic Bcl11b expressing DN2 progenitors, which have one active and one inactive Bcl11b allele and 3) biallelic Bcl11b expressing DN2 progenitors, which have both Bcl11b alleles active Overall design: Examination of H3K27me3 in hematopoietic progenitors that have no Bcl11b expression, monoallelic Bcl11b expression, and biallelic Bcl11b expression

本研究报道了不同分化阶段造血祖细胞（hematopoietic progenitor cells）的H3K27me3（H3K27me3）的CUT&RUN测序（CUT&RUN sequencing）数据集，所涉分化阶段包括：1）骨髓来源的cKit阳性造血祖细胞；2）表达单等位Bcl11b的DN2祖细胞，此类细胞仅一条Bcl11b等位基因处于活化状态，另一条则处于失活状态；3）表达双等位Bcl11b的DN2祖细胞，此类细胞的两条Bcl11b等位基因均处于活化状态。整体实验设计：分析无Bcl11b表达、单等位Bcl11b表达及双等位Bcl11b表达的造血祖细胞中的H3K27me3修饰水平。