Identification of circulating microRNAs as potential biomarkers for detecting acute ischemic stroke

In order to determine the serum microRNAs profile from middle-old aged patients with acute ischemic stroke and investigate possible diagnostic value of these differential microRNAs.The blood samples of 117 IS patients and 82 healthy people were collected. Differential miRNAs in serum from IS and control were screened with miRNA microarray analysis, and the expression of selected miRNAs were validated by quantitative reverse-transcriptase polymerase chain reaction assays (qRT-PCR). Results: We discovered 115 differentially expressed miRNAs, among which miR-32-3p, miR-106-5p, miR-532-5p were found be related to IS for the first time. Conclusions: In the present study, we identified the changed expression pattern of miRNAs in IS. Serum miR-32-3p, miR-106-5p, miR-1246 and miR-532-5p may serve as potential diagnostic biomarkers for IS. During the initial screening stage, we divided the serum samples into five groups (10 serum samples were pooled to form a group). Group A1: A denotes thrombotic stroke and 1 denotes hepertension. Group A14: A denotes thrombotic stroke, 1 denotes hepertension and 4 denotes hyperlipidemia. Group B2: B denotes embolic stroke and 2 denotes heart disease. Group B12: B denotes embolic stroke, 1 denotes hepertension and 2 denotes heart disease. Group 0: healthy control group.

本研究旨在明确中老年急性缺血性脑卒中（acute ischemic stroke, AIS）患者的血清微小核糖核酸（microRNAs, miRNAs）表达谱，并探讨此类差异表达miRNAs的潜在诊断价值。本研究共收集117例IS患者及82名健康对照者的血液样本。采用miRNA微阵列分析技术筛选IS患者与健康对照者血清中的差异表达miRNAs，并通过定量逆转录聚合酶链反应（qRT-PCR）验证目标miRNAs的表达水平。结果：本研究共筛选得到115个差异表达miRNAs，其中miR-32-3p、miR-106-5p及miR-532-5p为首次被发现与IS相关。结论：本研究明确了IS患者血清中miRNAs的异常表达模式，血清miR-32-3p、miR-106-5p、miR-1246及miR-532-5p有望成为IS的潜在诊断生物标志物。初筛阶段，本研究将血清样本分为5组（每10份血清样本混合为1个组）。具体分组如下：A1组：A代表血栓性脑卒中，1代表合并高血压；A14组：A代表血栓性脑卒中，1代表合并高血压，4代表合并高脂血症；B2组：B代表栓塞性脑卒中，2代表合并心脏疾病；B12组：B代表栓塞性脑卒中，1代表合并高血压，2代表合并心脏疾病；0组：健康对照组。