Effect of rivaroxaban on the collagen-stimulated PDGF-AB secretion from human platelets.

PRP was pretreated with 1000 ng/ml of rivaroxaban or vehicle for 15 min, and then stimulated by 1.0 μg/ml collagen or vehicle for 5 min. The reaction was terminated by the addition of ice-cold EDTA (10 mM) solution. The conditional mixture was centrifuged at 10,000 x g at 4°C and the supernatants were then subjected to an ELISA for PDGF-AB. Values for unstimulated platelets have been subtracted from each data point. Results from ten healthy donors are shown. Each value represents the mean ± SEM. N.S. designates no significant difference between the indicated pairs.

将富血小板血浆（PRP）用1000 ng/ml的利伐沙班或溶媒对照预处理15分钟，随后以1.0 μg/ml的胶原蛋白或溶媒对照刺激5分钟。反应通过加入冰浴冷却的10 mM乙二胺四乙酸（EDTA）溶液终止。将反应混合液在4℃、10000×g条件下离心，取上清液用于血小板衍生生长因子AB（PDGF-AB）的酶联免疫吸附测定（ELISA）。所有数据点均已扣除未受刺激的血小板组的对应数值。本实验展示了10名健康供体的实验结果，所有数值均以平均值±标准误（SEM）表示。"N.S."表示所标注的组间无显著性差异。