Dataset related to the article "Multiplexed MRM-Based Proteomics Identified Multiple Biomarkers of Disease Severity in Human Heart Failure"

This record contains raw data related to the article "Multiplexed MRM-Based Proteomics Identified Multiple Biomarkers of Disease Severity in Human Heart Failure"

Abstract

Heart failure (HF) is a complex disease due to the intricate interplay of several mechanisms, which therefore implies the need for a multimarker strategy to better personalize the care of patients with HF. In this study, we developed a targeted mass spectrometry approach based on multiple reaction monitoring (MRM) to measure multiple circulating protein biomarkers, involved in cardiovascular disease, to address their relevance in the human HF, intending to assess the feasibility of the workflow in the disease monitoring and risk stratification. In this study, we analyzed a total of 60 plasma proteins in 30 plasma samples from eight control subjects and 22 age and gender- matched HF patients. We identified a panel of four plasma proteins, namely
Neuropilin-2, Beta 2 microglobulin, alpha-1-antichymotrypsin, and complement component C9, that were more abundant in HF patients in relation to disease severity and pulmonary dysfunction.
Moreover, we showed the ability of the combination of these candidate proteins to discriminate, with sufficient accuracy, HF patients from healthy subjects. In conclusion, we demonstrated the feasibility and potential of a proteomic workflow based on MRM mass spectrometry for the evaluation of multiple proteins in human plasma and the identification of a panel of biomarkers of HF severity.

本数据集包含与题为《基于多重反应监测（multiple reaction monitoring, MRM）的蛋白质组学鉴定人类心力衰竭疾病严重程度相关多标志物》的学术论文相关的原始数据。

摘要

心力衰竭（Heart Failure, HF）是一种由多种机制复杂相互作用引发的复杂疾病，因此亟需采用多标志物策略以更好地实现心力衰竭患者的个体化诊疗。本研究开发了一种基于多重反应监测（MRM）的靶向质谱分析方法，用于检测多种参与心血管疾病的循环蛋白质标志物，以探究其在人类心力衰竭中的相关性，旨在评估该实验流程用于疾病监测与风险分层的可行性。本研究共分析了8名健康对照受试者与22名年龄、性别匹配的心力衰竭患者的30份血浆样本中的60种血浆蛋白。我们鉴定出一组共4种血浆蛋白，即神经纤毛蛋白-2（Neuropilin-2）、β2微球蛋白（Beta 2 microglobulin）、α1-抗糜蛋白酶（alpha-1-antichymotrypsin）以及补体成分C9（complement component C9），它们在心力衰竭患者体内的表达水平随疾病严重程度与肺功能障碍程度升高。此外，我们证实这些候选蛋白的组合能够以足够高的准确度区分心力衰竭患者与健康受试者。综上，本研究证明了基于MRM质谱的蛋白质组学实验流程在检测人类血浆中多种蛋白质以及鉴定心力衰竭严重程度相关标志物组方面的可行性与应用潜力。