TP53 knock-down in cultured, starved human adipocytes. TP53 knock-down in cultured, starved human adipocytes

The goal of this cell culture experiment was to validate in vivo data showing adipocyte p53-dependent changes of the immune cell landscape of visceral adipose tissue in obese mice that are interemittently fasted. Overall design: Therefore, human SGBS preadipocytes were differentiated into mature adipocytes before TP53 was knocked down via siRNA transfection. twentyfour hours before harvest, the cells were switched to starvation medium (HBSS+HEPES). Control cells were transfected with non-targeting siRNA and also starved. Each group contains triplicate measurements.

本细胞培养实验旨在验证体内实验数据，该数据揭示了在间歇性禁食的肥胖小鼠中，脂肪细胞p53依赖性地改变内脏脂肪组织的免疫细胞谱。实验设计概述：研究人员首先将人SGBS前脂肪细胞诱导分化为成熟脂肪细胞，随后通过siRNA（小干扰RNA）转染技术敲低TP53基因。在收获细胞前24小时，将细胞更换为饥饿培养基（HBSS+HEPES）。对照组细胞转染非靶向siRNA，并同步进行饥饿处理。每组均设置三次重复检测。