RNA sequencing

HT29 cells were transfected with NC plasmid and CHL1 overexpression plasmid, respectively (named HTNC and HTCHL1). SW480 cells were transfected with NC plasmid and CHL1 overexpression plasmid, respectively (named SWNC and SWCHL1). To study the mechanism of CHL1 overexpression on colorectal cancer cells.

将HT29细胞分别转染阴性对照（negative control, NC）质粒与CHL1过表达质粒，分别命名为HTNC与HTCHL1；将SW480细胞分别转染阴性对照质粒与CHL1过表达质粒，分别命名为SWNC与SWCHL1。本实验旨在探究CHL1过表达对结直肠癌细胞的作用机制。