Oryza sativa cultivar:Nipponbare Transcriptome or Gene expression. Oryza sativa cultivar:Nipponbare

Seeds of Oryza sativa (Nipponbare) were surface-sterilized with 20% bleach and 0.05% Tween-20 before treatments. Seeds (~30-50 individuals) were placed on petri dishes with wet filter paper and then were transferred to air control or hypoxia conditions under complete darkness. The hypoxia treatment was carried out using the Whitley H35 Hypoxystation (Don Whitley Scientific Limited, UK) with 3% O2 level at 28°C. Seed samples were harvested at 6 h after treatments and used for further transcriptomic and proteomic analysis.

水稻（Oryza sativa）日本晴（Nipponbare）种子在实验处理前，先用20%漂白剂与0.05%吐温-20（Tween-20）完成表面灭菌。取约30~50粒种子置于铺有湿润滤纸的培养皿中，随后转移至完全黑暗环境下的空气对照或低氧（hypoxia）条件中。低氧处理采用英国唐惠利科学有限公司（Don Whitley Scientific Limited, UK）生产的Whitley H35低氧工作站（Whitley H35 Hypoxystation），设置氧浓度为3%，培养温度为28℃。于处理后6小时收取种子样本，用于后续的转录组学（transcriptomic）与蛋白质组学（proteomic）分析。