Elucindating the role of the transcription factor IRF4 in Th17 and Treg cells

- transcription factor interferon regulatory factor 4 (IRF4) = crucial transcription factor for different immune cells, incl pro-inflammatory Th17 and anti-inflammatory Treg cells
- IRF4 is essential for the cell differentiation and fate determination
- however molecular mechanisms of IRF4-mediated gene expression in fully differentiated Th17/Treg cells are still not fully understood
- integration of data derived from affinity-purification and full mass spectrometry-based proteome analysis with chromatin immune precipitation sequencing (ChIP-Seq)
- characterization of proteins generally involved in the T cell development as well as subtype-specific differentiation and identification of novel, yet uncharted IRF4 interactors

- 转录因子干扰素调节因子4（interferon regulatory factor 4，IRF4）是调控多种免疫细胞的关键转录因子，涵盖促炎性Th17细胞与抗炎性Treg细胞
- IRF4对于细胞分化与细胞命运决定至关重要
- 然而，IRF4介导成熟分化的Th17/Treg细胞内基因表达的分子机制仍未完全阐明
- 本研究整合了亲和纯化结合全质谱蛋白质组分析所得数据与染色质免疫沉淀测序（chromatin immune precipitation sequencing，ChIP-Seq）的数据
- 旨在对参与T细胞发育及亚型特异性分化的蛋白进行特征分析，并鉴定出尚未被报道的新型IRF4互作蛋白