The role of extracellular vesicle miRNAs and tRNAs in synovial fibroblast senescence. The role of extracellular vesicle miRNAs and tRNAs in synovial fibroblast senescence

We studied extracellular vesicles (EVs) from synovial fibroblast (SF). EVs were isolated from the secretome of non-senescent and irradiation-induced senescent SFs using size exclusion chromatography. EV RNA was extracted and subject to small RNA sequencing on an Illumina NovaSeq SP using 100bp, single end reads. After mapping against GRCh38.p12 and miRBase v22.1 differential expression analysis was undertaken with edgeRv3.28 using a quasi-likelihood negative binomial generalized log-linear model. We identified a panel of differentially expressed small non-coding RNAs. Overall design: Examination of small non-coding RNAs of extracellular vesiscles derived form the secretome of synovial fibroblasts from osteoathritic joint tissue using pairwise comparisons.

我们针对滑膜成纤维细胞（synovial fibroblast, SF）来源的细胞外囊泡（extracellular vesicles, EVs）开展了相关研究。本研究通过尺寸排阻色谱法，从非衰老及辐照诱导衰老的滑膜成纤维细胞分泌组中分离纯化细胞外囊泡；随后提取细胞外囊泡RNA，采用Illumina NovaSeq SP平台，以100bp单端读长进行小RNA测序。将测序数据比对至人类参考基因组GRCh38.p12及miRBase v22.1数据库后，借助edgeRv3.28软件，基于拟似然负二项广义对数线性模型开展差异表达分析，最终筛选得到一组差异表达的小非编码RNA。整体实验设计：通过两两比较，分析骨关节炎关节组织来源的滑膜成纤维细胞分泌组所衍生的细胞外囊泡中的小非编码RNA。