Autophagy mediates the mitotic senescence transition. Homo sapiens

ER:RAS-G12V expressing IMR90 cells were given 100nM 4-OHT and assessed for transcriptional profiling at various time points. Overall design: IMR90 cells expressing a 4-hydroxytamoxifen (4-OHT) inducible estrogen receptor (ER)-coupled RAS-G12V (ER:RAS-G12V) were given 100nM 4-OHT for these eight time points (0, 2, 8, 24hrs, 2, 4, 6 and 8days) then analysed for transcriptional profiling using the illumina expression array platform (human-6 v2 Expression BeadChips). There were three biological replicates (batch A, B and D) for each of the time points.

本数据集针对表达ER:RAS-G12V的IMR90细胞展开研究：以100nM 4-羟基他莫昔芬（4-hydroxytamoxifen，4-OHT）处理细胞后，于8个时间点（0、2、8、24小时，2天、4天、6天、8天）开展转录谱分析。具体实验设计如下：将表达4-羟基他莫昔芬可诱导型雌激素受体（estrogen receptor，ER）偶联RAS-G12V（ER:RAS-G12V）的IMR90细胞，针对上述时间点施加100nM 4-OHT处理，随后采用Illumina表达芯片平台（Human-6 v2 Expression BeadChips）进行转录谱分析。每个时间点均设置3个生物学重复（批次A、B、D）。