Antibiotic-responsive microbial transcriptomes

RNA-Seq on common bacterial pathogens in the presence vs absence of a variety of antibiotics. Two susceptible and two resistant isolates were exposed to antibiotics in laboratory culture, total RNA was extracted at various timepoints from cultures with and without antibiotics, and cDNA libraries were prepared for Illumina sequencing.

针对常见细菌性致病菌在多种抗生素存在与缺失条件下的RNA测序（RNA-Seq）实验：选取2株敏感分离株与2株耐药分离株，在实验室培养体系中施加抗生素处理；分别于不同时间点从添加与未添加抗生素的培养物中提取总RNA，并构建互补脱氧核糖核酸（cDNA）文库以开展Illumina测序。